Last data update: May 06, 2024. (Total: 46732 publications since 2009)
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PCR-based method for S. flexneri serotyping: International Multicenter Validation.
Brengi SP , Sun Q , Bolanos H , Duarte F , Jenkins C , Pichel M , Shahnaij M , Sowers EG , Strockbine N , Talukder KA , Derado G , Vinas MR , Kam KM , Xu J . J Clin Microbiol 2019 57 (4) Shigella is a leading cause of human diarrheal disease worldwide with S. flexneri being the most frequently isolated in developing countries. This serogroup is presently classified into 19 serotypes worldwide. We report here a Multicenter Validation of a Multiplex PCR based-strategy previously developed by Sun et al. (doi: 10.1128/JCM.01259-11) for molecular serotyping of S. flexneri This study was performed by seven international laboratories, with a panel of 71 blinded strains as well as 279 strains collected from each own local culture collections. This collaborative work found high extent of agreement among laboratories, calculated through IRR measures for PCR test, proven its robustness. It was also observed agreement with traditional method (serology) in all laboratories for 14 serotypes studied, while specific genetic events could be responsible for the discrepancies among methodologies in other five serotypes as determined by PCR product sequencing in most of the cases. This work provided an empirical frame that allowed the use of this molecular method to serotype S. flexneri, and showed several advantages over the traditional method of serological typing. These included overcoming the problem of availability of suitable antisera in testing laboratories, as well as facilitated the analysis of multiple samples at the same time. The method is also less time consuming for completion, and easier to implement in routine laboratories. We recommend that this PCR be adopted, as it is a reliable diagnostic and characterization methodology that can be used globally for laboratory-based shigellae surveillance. |
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